Abstract
Changes in bioelectrical activity of nerve cells after their long-term exposure to autoblood were studied in vitro on cultured brain slices. This model simulated the events characteristic of a hemorrhagic stroke. Brain slice was placed into a glass vial with autoblood for 60-420 min, after which the slice was transferred into a perfusion chamber and after washing from autoblood their focal potentials were recorded. The level and reversibility of disorders in nerve cell activity were detected by comparing the parameters of focal potentials with the control values. Delayed effects of autoblood were detected, manifesting in the progress in disorders of nerve cell activity with prolongation of exposure in the blood, and the period after which they could be restored was determined.
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