Abstract
Results from our previous study proved that differential scanning calorimetry (DSC) is a reliable method for qualitative evaluation of morphological changes in human cartilage samples caused by cryopreservation and storage time correlates with graft quality [1, 2]. Our current aim was to specify/characterise the effects of deep-freezing on hyaline cartilage in conjunction with length of storage time. Detailed analysis included DSC and histological measurements which showed distinctive differences. Based on our histological examinations, we could not confirm significant association between cartilage destruction and long-time storage, but the DSC results exhibited a definite change in thermal parameters after the 6th week cryopreservation. A modified and more detailed analysis would be useful to determine the effects of deep-freezing.
Highlights
Pathologic lesions affecting articular cartilage and subchondral bone such as traumatic focal osteochondritis dissecans (OCD) and patella chondromalacia mostly affect young and physically active age groups
In fresh allografts stored at 4–6 °C, signs of permanent damage to chondrocytes were already detectable after 21 days [5]
The most remarkable change caused by the freezing is a Tm around 85 °C after the first week storage compared to the control sample
Summary
Pathologic lesions affecting articular cartilage and subchondral bone such as traumatic focal osteochondritis dissecans (OCD) and patella chondromalacia mostly affect young and physically active age groups. Consequences in these conditions include progressive degeneration of articular cartilage (osteoarthritis), early retirement from an athletic career, and lower level of regular/daily physical activity. Osteochondral allograft transplantation (OAC) has become a valid solution for the problems associated with autologous mosaicplasty. It provides a viable alternative for total pain relief and functional recovery after cartilage restoration.
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