Effects of long-term conservation tillage on N2 and N2O emission rates and N2O emission microbial pathways in Mollisols

Abstract

Conservation tillage is widely used in farmland management for soil carbon sequestration, but it can also lead to potential emissions of nitrous oxide (N2O). Therefore, our study is aimed to investigate the effects of 15 years of no-tillage combined with four straw mulching levels of 0 % (NT0), 33 % (NT33), 67 % (NT67), and 100 % (NT100) compared to ridge tillage (RT) on the rates of N2O and N2 emissions and the respective contributions of four microbial pathways to N2O emissions. The incubation experiments were conducted at two different moisture levels (55 % and 100 % WFPS) by using dicyandiamide inhibition and 15N-labeling techniques. Soil samples were collected from the 0–20 cm and 20–40 cm soil depths across three maize growth stages: seedling, jointing, and maturity. Our results showed that conservation tillage significantly decreased the N2O + N2 emission at 55 % WFPS, but it has a reverse influence in N2O + N2 emission at 100 % WFPS. The proportion of N2O in gaseous N loss were higher at 100 % WFPS than at 55 % WFPS. Among the four microbial pathways for N2O emissions, autotrophic nitrification was the dominant pathway 55 %WFPS. The contribution of autotrophic nitrification remarkably decreased, co-denitrification and denitrification increased at 100 %WFPS. Overall, at 100 % WFPS, N2O emissions from all major microbial pathways were positively correlated with GWC, temperature, TC, TN, NH4+-N, and NO3−-N, but negatively correlated with soil pH and C/N ratios. Our results suggest that long-term conservation tillage increases N2O and N2 emissions from the soil under water-saturated conditions by regulating soil nutrient levels, soil moisture, and microbial pathways. Therefore, we should consider the impact of conservation tillage on N2O emission risk when we attach importance to the role of conservation tillage in improving soil quality and increasing crop yields.