Effects of long noncoding RNA TUG1 on migration and invasion of osteosarcoma cells

Abstract

Objective To study the effects of TUG1 on migration and invasion of osteosarcoma cells. Methods The osteosarcoma Saos-2 cells were classified into three groups: TUG1 small interfering RNA (siRNA group), blank control group (Con-A), and the negative control-siRNA group (Con-B). The transfection reagent oligofectamine was mixed with TUG1 siRNA and nonsense siRNA, respectively. The TUG1 knocking down effects were examined by real-time polymerase chain reaction (PCR). The migration of osteosarcoma cells was determined by wound healing test, and the invasion ability was studied by Transwell assay. Results As compared with Con-A and Con-B groups, the expression level of TUG1 mRNA was decreased in siRNA group. After osteosarcoma cells were transfected with TUG1 siRNA, the results of wound healing test showed that the space between scratches of Con-A, Con-B and siRNA groups was (326±11) μm, (335±15) μm, and (588±11) μm respectively (F=22.761, P=0.000). The results of the Transwell showed that the membrane cell number in Con-A, Con-B and siRNA groups was (125.0±2.2), (118.0±1.8), and (56.0±1.8) cells respectively (F=173.000, P=0.000). Conclusion TUG1 might be associated with migration and invasion of osteosarcoma cells. Key words: Osteogenic sarcoma; Long noncoding RNA; TUG1; Migration; Invasion