Effects of Loligo Squid Ink on Sexual Arousal Parameters in D-Galactose-Induced Aging Male Mice

Abstract

Squid ink is known to contain many bioactive properties such as antioxidant, anticancer activity, antimicrobial activity, and anti-inflammatory activity. Through its antioxidant properties squid was suggested to have therapeutic potential for sexual function disorders. This study is an attempt to evaluate whether squid ink has therapeutic benefits against age-related decreases in sexual arousal in D-galactose-induced aging male mice. Four groups of male mice were treated as follows. Group-1 received only standar food but no D-galactose induction nor squid ink given. Group-2 received D-galactose induction but not squid ink. Group-3 and group-4 received D-galactose induction and squid ink at the dose of 40 and 100 ml/kg body weight respectively. After 35 days of treatment, the test mice were mated with estrous virgin females to determine their levels of sexual arousal. The results, Loligo squid ink extract at a dose of 100 ml/kg body weight significantly enhances sexual arousal parameter values in the D-galactose-induced aging mouse model. However, the recovery rate did not achieve nor surpassed the parameter values of sexual arousal in normal mice. It suggests that Loligo squid ink has the potential to prevent damage to sexual function in males but is less effective in restoring it.