Effects of LncRNA BC168687 siRNA on Diabetic Neuropathic Pain Mediated by P2X7 Receptor on SGCs in DRG of Rats.

Chenglong Liu,Jia Tao,Yixin Yang,Changshui Xu,Jiandi Liu,Zeyu Deng,Qiang Chen,Hui Wu

doi:10.1155/2017/7831251

Abstract

Diabetic neuropathic pain (DNP), one of the early symptoms of diabetic neuropathy, relates to metabolic disorders induced by high blood glucose, neurotrophic vascular ischemia and hypoxia, and autoimmune factors. This study was aimed at exploring the effects of long noncoding RNA (lncRNA) BC168687 siRNA on DNP mediated by P2X7 receptor on SGCs in DRG of rats. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of rats, the expression levels of P2X7 mRNA and protein in the DRG, and nitric oxide (NO) in the serum were, respectively, detected in our study. Our experimental results showed that the level of BC168687 mRNA in DNP group was markedly higher than that of control group; the MWT and TWL of DNP + BC168687 si group were significantly increased, and the expression levels of P2X7 in DRG and the concentrations of NO in serum of DNP + BC168687 si group were decreased compared to those of the DNP group. In conclusion, lncRNA BC168687 may participate in the pathogenesis of DNP mediated by P2X7 receptor, which will provide a novel way for the study of the pathogenesis of diabetes mellitus complicated with neuropathic pain and its prevention and treatment.

Highlights

Long noncoding RNA (LncRNA), the cognition of which is still unclear, is a byproduct of RNA polymerase II transcription and has no biological function [1]
Our experimental results revealed that the level of long noncoding RNA (lncRNA) BC168687 in DRG of the Diabetic neuropathic pain (DNP) group was significantly higher than that of the control group by reverse transcription-PCR (Figure 1), indicating that lncRNA BC168687 was involved in the initiation of DNP in rats
There was an interaction between time factors and treatment factors in the mechanical withdrawal threshold (MWT) (F8,525 = 3.050, P < 0.01) and the thermal withdrawal latency (TWL) (F8,525 = 4.209, P < 0.01); after intrathecal injection with BC168687 siRNA, the MWT and TWL of each group increased on the 1st, 3rd, and 5th days, indicating that BC168687 siRNA contributed to alleviating DNP

Summary

Introduction

Long noncoding RNA (LncRNA), the cognition of which is still unclear, is a byproduct of RNA polymerase II transcription and has no biological function [1]. LncRNAs could produce a complex regulatory process by interacting with transcription factors, coactivators, and/or inhibitory factors to influence various aspects of gene transcription [4, 5]. Studies have demonstrated that knockout of mice lncRNAs can lead to functional abnormalities [6, 7]. DNP has severe effects on the quality of patients’ life and could bring immense physical and mental suffering to the patients. Statistics indicate that the number of patients suffering from DNP accounts for 50%. Growing evidence supports that people with components of the metabolic

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