Abstract
目的 通过不同途径行Sertoh细胞-肝脏联合移植,探讨Sertoli细胞是否可为移植肝提供免疫保护.方法 2步法分离培养Sertoli细胞,二袖套管法行大鼠原位肝移植并以Wistar→SD组合建立排斥反应模型.通过三种途径进行Sertoli细胞-肝脏联合移植.分别观察术后各组症状、体征、肝功能变化、移植肝病理特征等.采用免疫组化、凋亡等技术检测Sertoli细胞功能及作用,探讨其对肝移植急性排斥的影响.结果 肝移植急排模型不干预组14只,1只存活超过14 d.Settoli细胞腹腔注射组、阴茎背静脉注射组和供体移植前门静脉注射组分别有5、8、7只存活超过14 d.各干预组存活率与对照组比较:后两组差异有显著性(P＜0.05),腹腔注射组差异不显著(P＞0.05);各干预组之间存活率差异无显著性(P＞0.05).供肝病理检查显示各干预组排斥反应较对照组轻.免疫组化及凋亡检测发现:肝移植后14 d,Sertoli细胞仍存活并表达FasL,Sertoli细胞周围有淋巴细胞集聚及凋亡的淋巴细胞.结论 Sertoli细胞对肝移植急性排斥有抑制作用,对供肝有诱导免疫耐受作用,Sertoli细胞通过Fas/FasL途径诱导淋巴细胞凋亡。
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