Abstract
Studies were made on the effects of Li + on ADP ribosylation of inhibitory GTP-binding (G 1) protein by islet-activating protein (IAP), pertussis toxin. The ADP ribosylation of 40–41 kDa proteins of the membranes of rat cardiac ventricular cells by IAP was reduced by the addition of a nonhydrolyzable analog of guanine nucleotide. GTPγS, indicating that these proteins included G 1 protein. The addition of LiCl (0.5–10 mM) to the membrane fractions of the cells attenuated the ADP ribosylation of the G i protein of the cell membranes by IAP dose-dependently. The effects of LiCl were reversible. Of the monovalent ions tested. Li + inhibited the ADP ribosylation of the protein by IAP most strongly. The effects of LiCl (2 mM) were observed even in the presence of 150 mM KCl. Moreover, LiCl decreased the ADP ribosylation of purified G 1 protein by IAP. These results support that G 1 proteins are one of the targets for the therapeutic effects of lithium.
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