Abstract
No systematic investigation considering BDNF and its receptor TrkB has been conducted on the effects of mood-stabilizing drugs. We therefore decided to quantify BDNF production and the expression of TrkB-T1 receptor and PLCγ in astrocytoma. 1321N human astrocytoma cells were grown to a sufficient quantity in 5% fetal calf serum (FCS). The mood-stabilizing drugs Li and VPA were added to the therapeutic concentrations of 1 mM and 600 μM, respectively. The production of BDNF was determined by an enzyme-linked immunosorbent assay. The expressions of TrkB-T1 and PLCγ were determined by Western blot. The production of BDNF was significantly higher on Day 7 in the VPA samples (P γ expression did not change. Li and VPA seem differently affect BDNF production and TrkB-T1 (BDNF receptor) expression.
Highlights
Bipolar disorder, a manic-depressive disease, persists throughout life in 0.8% of those afflicted [1]
The production of brain-derived neurotrophic factor (BDNF) did not differ in the control and valproic acid (VPA) samples to Day 5, but was significantly suppressed from Days 1 to 7 in the Li samples (p < 0.05)
The expression of TrkB-T1 in the groups did not differ on Day 1 (Figure 2), but was significantly higher in the VPA group beginning on Day 3 (p < 0.05)
Summary
A manic-depressive disease, persists throughout life in 0.8% of those afflicted [1]. Refractory to treatment, bipolar disorder often recurs repeatedly This serious disease is sometimes marked by rapid cycling between manic-depressive episodes, which hinders the social activities of patients. Lithium (Li) and valproic acid (VPA), are the primary treatment for bipolar disorder. Lithium was first shown to be effective in bipolar disorder by Schou et al, and is still a firstline treatment [3]. The mechanism of VPA shares commonalities with Li, such as its actions on the inositol phospholipids and effects on GSK-3β and bc1-2 [7], but there is a paucity of data to assist with the selection of mood-stabilizing drugs in the clinic because many aspects of the mechanisms of action of these two mood-stabilizing drugs remain unclear
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
