Effects of Liquid Probiotics (Lactobacillus sp.) on Microflora Balance, Enzyme Activity, Number and Surface Area of the Intestinal Villi of Broiler

Muhammad Izzuddiyn,Woro Busono,Osfar Sjofjan

doi:10.21776/ub.jpal.2018.009.02.04

Muhammad Izzuddiyn, Woro Busono + Show 1 more

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https://doi.org/10.21776/ub.jpal.2018.009.02.04

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Abstract

The aims of this study were to determine the effects of probiotic liquid as feed additive on productivity, microflora balance, enzym activity, number and surface of intestinal villi of broiler. This study was used experimental method with Completely Randomized Design (CRD) on 144 Day Old Chicks (DOC) Lohman strain, which divided into 4 treatment groups and 6 replications. The treatment was the basal feed without the addition of liquid probiotic (P0); basal feed with 0,2 v/w liquid probiotic (P1); basal feed with 0,4 v/w liquid probiotic (P2) and basal feed with 0,6 v/w liquid probiotic (P3). Liquid probiotic containing composition of Lactobacillus sp. 1,4x10 10 cfu/mLl. Data were analyzed using a CRD followed by the Duncan Multiple Range Test. The results showed that liquid probiotic ( Lactobacillus sp.) as food additive give the significant effect (P 0,05) to protease and lipase activity. It`s showed significant effect (P<0,01) to total plate count (TPC) of lactid acid bacteria, Salmonella sp., Eschericia coli , surface of intestinal villi.. The result were concluded that the higher concentration of liquid probiotics in feed the higher microflora balance, protease and lipase activity, number and surface of intestinal villi. Decreasement of Salmonella sp. and Eschericia coli TPC. As suggestion, a liquid probiotics ( Lactobacillus sp.) 0.6 v/w in feed can produce the higher performance. Keywords: broilers, intestinal characteristics, liquid probiotic

Highlights

Use of antibiotics in broilers besides to expected positive impacts but can get negative impacts, among others, stimulate the emergence of bacterial resistance to these antibiotics and cause residue in the meat
The effect of probiotics associated with bacterial populations in regulating the balance of intestinal microflora can be determined by counting the total number of viable counts (TVC) and total lactobacillus count (TLC) of the cecum samples given probiotics
Number of Lactic Acid Bacteria Colonies (BAL) The mean values in Table 3 showed that the highest number of BAL colonies is P3 while the lowest is P0

Summary

Introduction

Use of antibiotics in broilers besides to expected positive impacts but can get negative impacts, among others, stimulate the emergence of bacterial resistance to these antibiotics and cause residue in the meat. The emergence of bacterial resistance can have an effect on reducing the highly beneficial microflora in the intestine, whereas the presence of antibiotic residues can have an allergic or hypersensitivity impact which is one of the precursors of cancer. On this basis, many countries reject animal foods derived from livestock fed antibiotics [1]. Probiotics play a role to improve the balance of intestinal microflora and increase the number of beneficial microbes so that pathogenic bacteria inhibited. Probiotics play a role in inhibiting the colonization of pathogenic bacteria by forming a block of digestive coating and removal of pathogen bacteria attached to the intestinal

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