Effects of liquefied sake lees on growth performance and faecal and blood characteristics in Japanese Black calves.

Abstract

Liquefied sake lees, a by-product of Japanese sake, is rich in Saccharomyces cerevisiae, proteins, and prebiotics derived from rice and yeast. Previous studies have reported that Saccharomyces cerevisiae fermentation products improved the health, growth, and faecal characteristics of preweaning calves. This study investigated the effects of adding liquefied sake lees to milk replacer on the growth performance, faecal characteristics, and blood metabolites of preweaning Japanese Black calves from 6 to 90days of age. Twenty-four Japanese Black calves at 6days of age were randomly assigned to one of three treatments: No liquefied sake lees (C, n=8), 100g/d (on a fresh matter basis) liquefied sake lees mixed with milk replacer (LS, n=8), and 200g/d (on a fresh matter basis) liquefied sake lees mixed with milk replacer (HS, n=8). The intake of milk replacer and calf starter, as well as, the average daily gain did not differ between the treatments. The number of days counted with faecal score 1 in LS was higher than in HS (P<0.05), while the number of days with diarrhoea medication in LS and C was lower than HS (P<0.05). The faecal n-butyric acid concentration tended to be higher in LS compared to C (P=0.060). The alpha diversity index (Chao1) was higher in HS than in C and LS at 90days of age (P<0.05). The principal coordinate analysis (PCoA) using weighted UniFrac distance showed that the bacterial community structures in faeces among the treatments at 90days of age were significantly different (P<0.05). The plasma β-hydroxybutyric acid concentration, an indicator of rumen development, was higher for LS than in C throughout the experiment (P<0.05). These results suggested that adding liquefied sake lees up to 100g/d (on a fresh matter basis) might promote rumen development in preweaning Japanese Black calves.