Effects of Lipid Peroxidation-Mediated Ferroptosis on Severe Acute Pancreatitis-Induced Intestinal Barrier Injury and Bacterial Translocation.

Deliang Ma,Yingjian Jiang,Dianliang Zhang,Hongbo Li,Pengling Jiang,Ana Cipak Gasparovic

doi:10.1155/2021/6644576

Deliang Ma, Yingjian Jiang + Show 4 more

Open Access

https://doi.org/10.1155/2021/6644576

Copy DOI

Abstract

Ferroptosis is a recently recognized type of regulated cell death characterized by iron- and lipid peroxidation-mediated nonapoptotic cell death. However, whether ferroptosis is involved in severe acute pancreatitis- (SAP-) induced intestinal barrier injury is unknown. The aim of this study was to investigate whether ferroptosis is involved in SAP-induced intestinal barrier injury, particularly intestinal epithelial cell (IEC) death, and determine whether the inhibition of ferroptosis would ameliorate intestinal barrier injury and prevent bacterial translocation (BT). Sodium taurocholate (5%) was retrogradely perfused into the biliopancreatic duct to establish a rat model of SAP. The rats were divided into three groups: sham operation (SO), SAP-induced intestinal barrier injury (SAP), and ferroptosis inhibitor liproxstatin-1 (SAP + Lip). Serum indexes were measured in the rats. In addition, the biochemical and morphological changes associated with ferroptosis were observed, including iron accumulation in intestinal tissue, lipid peroxidation levels, and mitochondrial shrinkage. Hematoxylin staining and eosin staining were used to assess histological tissue changes. Western blot, RT-PCR, and immunofluorescent staining were performed to analyze the expression of ferroptosis-related proteins and genes as well as tight junction. BT was detected by 16S rDNA sequencing analysis. The results indicated that ferroptosis was significantly induced in the IECs from rats with SAP and ferroptosis was mediated by lipid peroxidation. The specific lipid peroxidation of IECs clearly upregulated ferroptosis and exacerbated intestinal barrier injury. Furthermore, treatment with liproxstatin-1 lowered the levels of serum damage markers, decreased lipid peroxidation, and alleviated intestinal and acute remote organ injury in SAP rats. In addition, inhibition of ferroptosis reduced BT. Our findings are the first to demonstrate that ferroptosis contributes to SAP-induced intestinal barrier injury via lipid peroxidation-mediated IEC death. These results suggest that ferroptosis is a potential therapeutic target for SAP-induced intestinal barrier injury.

Highlights

Acute pancreatitis is a common acute abdominal condition with rapid onset, and it can lead to high mortality when it develops into severe acute pancreatitis (SAP) [1]
We report for the first time that ferroptosis is involved in the intestinal epithelial cell (IEC) death of severe acute pancreatitis- (SAP-)induced intestinal mucosal barrier dysfunction, which is mediated by lipid peroxidation
We confirmed for the first time that ironand reactive oxygen species-dependent ferroptosis is involved in IEC death in SAP-induced intestinal barrier injury

Summary

Introduction

Acute pancreatitis is a common acute abdominal condition with rapid onset, and it can lead to high mortality when it develops into severe acute pancreatitis (SAP) [1]. Secondary infection of the pancreas is considered to be the main cause of the high mortality of SAP [2]. The relationship between intestinal barrier dysfunction and SAP has been confirmed. SAP can destroy normal intestinal mucosal function, leading to bacterial and endotoxin translocation into systemic circulation and eventually leading to secondary infection, systemic inflammatory response syndrome, and multiple organ dysfunction syndrome [4, 5].

Objectives

Methods

Results

Conclusion