Abstract
ABSTRACT. Many studies of nutrient allocation to egg production in birds use stable isotope ratios of egg yolk to identify the origin of nutrients. Dry egg yolk contains >50% lipids, which are known to be depleted in 13C. Currently, researchers remove lipids from egg yolk using a chemical lipid-extraction procedure before analyzing the isotopic composition of protein in egg yolk. We examined the effects of chemical lipid extraction on δ13C, δ15N, and δ34S of avian egg yolk and explored the utility of an arithmetic lipid correction model to adjust whole yolk δ13C for lipid content. We analyzed the dried yolk of 15 captive Spectacled Eider (Somateria fischeri) and 20 wild King Eider (S. spectabilis) eggs, both as whole yolk and after lipid extraction with a 2:1 chloroform:methanol solution. We found that chemical lipid extraction leads to an increase of (mean ± SD) 3.3 ± 1.1‰ in δ13C, 1.1 ± 0.5‰ in δ15N, and 2.3 ± 1.1‰ in δ34S. Arithmetic lipid correction provided accurate values for lipid-extracted δ13C in...
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