Effects of linolenic acid on transient outward current in atrial myocytes from patients with heart failure

Abstract

To explore the effects of linolenic acid(LNA,C18:3 n 3)on transient outward current(I to ) in atrial myocytes from patients with heart failure.Patch clamp technique was used to record I to in atrial myocytes from patients with heart failure before and after administration of extracellular LNA.Results:①The LNA induced suppression of I to current shown dose dependent and frequency independent.The I to inhibition percent of 2.5,5 and 10 μmol/L LNA respectively were 16%±4%,28%±6% and 51%±6%( P0.05 ),②The activation curves from relative conductance in the absence and presence of LNA(5 μmol/L)almost overlapped.The 50% channel activation was at 18.2±1.7 and 17.0±1.8 mV in the absence and presence of 5 μmol/L LNA resepectively( P0.05 ).Steady state inactivation curve shifted to left from -32.8±2.6 to -45.4±3.4 mV significantly,in the absence and presence of 5 μmol/L at V 1/2 point( P0.01 ).③The time for 50% recovery delayed from 39.3±3.4 to 85.1±5.8 ms significantly in the absence and presence of 5 μmol/L LNA( P0.01 ).Conclusions:The present data demonstrated that linolenic acid suppressed I to and prolonged the duration of recovery from inactivation in atrial myocytes from patients with heart failure.