Effects of light intensity and temperature on photoautotrophic growth of a green microalga, Chlorococcum littorale

Abstract

A highly CO2-tolerant green alga, Chlorococcum littorale, was investigated at temperatures ranging from 8 to 28°C, light intensities from 30 to 170μmolm−2s−1, a constant CO2 concentration of 5% (v/v) and atmospheric pressure. The experimental results showed that a specific growth rate μ, defined in terms of cell growth rate under a logarithmic growth phase, increased with temperature up to the maximum value (ca. 22°C), while the μ decreased at higher temperatures. These promotion and inhibition of the cell growth rate were expressed by both a multiple linear regression and a mathematical model taking account of the Arrhenius activation/deactivation energies. Light intensity affected on the cell growth was independently treated in the mathematical model. The proposed growth model agreed well with the experimental data to within 6.6 %, which provides good correlation for both temperature and light intensity effects on the microalgal cell growth.