Abstract
BackgroundHaworthia are desert succulents belonging to the Asphodelaceae family. Haworthia species are cultivated commercially as ornamentals and some rare species are quite valuable at retail market but growth slowly and difficult to propagation. However, an efficient micropropagation protocol was remained insufficient.ResultsThe organogenic cultures obtained from inflorescence explants were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA) under a light intensity of 10 μmol m−2 s−1 or 45 μmol m−2 s−1. The highest callus proliferation index (93.15%) with 1.0 mg L−1 BA + 0.1 mg L−1 NAA under a light intensity of 10 μmol m−2 s−1. The best shoot proliferation rates were on media with either 1 mg L−1 BA + 0–0.4 mg L−1 NAA (65.57–81.01%) under a light intensity of 45 μmol m−2 s−1. The highest root length (15.57 mm) and the highest rooting frequency (17 roots per shoot) were obtained when adventitious shoots were inoculated on MS medium with 0.4 mg L−1 NAA + 0.4 mg L−1 IBA. The survival rate of the transplanted plantlets was about 100%. The efficient micropropagation protocol proliferated Haworthia regenerate plants from inflorescence within 11 weeks.ConclusionsThe present study determined the best combination of light intensity and plant growth regulators (PGRs) for improved organogenesis of Haworthia during propagation by tissue culture. This optimized protocol showed light intensity is an important factor for efficient callus or shoot regeneration. These results indicate that it will be useful to optimize the light conditions for future commercial cultivation, germplasm conservation, genetic engineering and molecular biology research of this ornamental plant.
Highlights
Haworthia are desert succulents belonging to the Asphodelaceae family
Plants in the genus Haworthia are perennial, monocotyledonous, desert succulents belonging to the Asphodelaceae family
Haworthia species are propagated from seed, leaf cutting, crown division, or the propagules produced on the inflorescence (Pilbeam 1983), all of require time
Summary
Haworthia are desert succulents belonging to the Asphodelaceae family. Haworthia species are culti‐ vated commercially as ornamentals and some rare species are quite valuable at retail market but growth slowly and difficult to propagation. Plants in the genus Haworthia are perennial, monocotyledonous, desert succulents belonging to the Asphodelaceae family. Micropropagation has been used to aid in the reproduction of some self-incompatible Haworthia species (Mycock et al 1997), through inducing regeneration from leaves (Beyl and Sharma 1983; Rogers 1993), inflorescences (Kaul and Sabharwal 1972; Majumdar 1970a) and ovary walls (Majumdar 1970b). The effect of light intensity on in vitro shoot proliferation of Haworthia species has not been reported
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