Effects of level of food intake on ovarian follicle number, size and steroidogenic capacity in the ewe.

Abstract

The effect of high (H) or low (L) levels of food intake, during the preceding 4 weeks, on ovarian follicle numbers and steroidogenic capacity were investigated in groups of 12 adult Scottish Blackface ewes. Ewes of the two treatments had similar levels of body condition at the time of study but there was a twofold difference in levels of food intake. Ovaries were surgically removed on day 11 or 12 of the oestrous cycle (luteal phase; n = 6 per nutritional treatment) or at 30 h after injection (i.m.) of prostaglandin F2alpha analogue on day 11 or 12 of the cycle (follicular phase; n = 6 per nutritional treatment). Ovarian follicles > 1 mm diameter were dissected out and incubated individually for 2 h at 37 degrees C, in 1 ml of medium 199 which was then assayed to determine concentrations of oestradiol and testosterone. There were significantly more small follicles (1-2.5 mm diameter) in (H) than (L) ewes (P < 0.05) but no treatment difference in the numbers of large follicles (> 2.5 mm diameter) during either phase of the cycle and no difference in the mean diameters (mm) of the two largest follicles in each animal. However, although there were higher rates of synthesis of both oestrogen (P < 0.05) and testosterone (P < 0.01) in the large follicles of (L) ewes as compared with (H) ewes, there was a lower oestrogen/testosterone ratio in (L) than (H) follicles which may indicate a lower level of aromatase activity in (L) follicles. It is concluded that the effects of level of food intake on ovulation rate are expressed through differences in late stages of follicle development, probably through effects on the intrafollicular steroid milieux.