Effects of lentiviral transfection containing bFGF gene on the biological characteristics of rabbit BMSCs.

Abstract

To investigate the effect of lentiviral transfection containing bFGF gene on the biological characteristics of bone marrow stromal cells (BMSCs). BMSCs were obtained by density gradient centrifugation and adherence screening. The bFGF gene was transfected into BMSCs by lentiviral vector and divided into bFGF transfection group, empty virus group, and untransfected group according to the transfection conditions. After transfection, the morphology, expression of bFGF mRNA and protein, cell proliferation, cell cycle, and alkaline phosphatase (ALP) activity were observed in three groups of cells. High density BMSCs were successfully obtained by density gradient centrifugation and adherence screening. After transfection of BMSCs with bFGF gene, the cell morphology did not change significantly, while the expression of bFGF mRNA and protein were significantly increased, the cell proliferation curve shifted upward, the proportion of proliferating cells increased, and the activity of ALP was significantly enhanced. Compared with empty virus group and untransfected group, there was significant difference (P < 0.05). The rabbit bFGF gene was successfully introduced into the BMSCs cultured in vitro by lentiviral vector, and the target gene was stably expressed. And the expression of bFGF can promote the proliferation and osteogenic differentiation of BMSCs.