Abstract
Summary. Ingestion of dried forage, incorporated into a Chow diet at a level of 15 or 30%, induced infertility and reproductive anomalies in female mice. Conception rate in SWR/Jax mice was nil during a 22-day cohabitation with fertile males, as opposed to 57% conception in controls. The fertility of CD-1 male mice was not affected by Ladino. Vaginal and behavioural oestrus in CD-1 mice was inhibited for 18 or more days during feeding of 30% Ladino clover, but animals gradually adjusted whilst ingesting clover, and after 18 days oestrus reappeared and fertile matings occurred. Ingestion of 15% prebloom Ladino inhibited oestrus, but oestrus was more frequent and persistent than normal in mice receiving 15% second-growth Ladino. Feeding 30% mixed-growth Ladino for only 3 days caused a severe reduction in number of fertilized ova recoverable and a concomitant increase in degenerate ova; longer feeding frequently inhibited ovulation and sexual receptivity in spite of vaginal cornification. No single aqueous-alcoholic, etheralcoholic, chloroform, or acetone-chloroform extract of forages had any of the peculiar properties of the unfractionated plant, but all extracts increased the incidence of non-viable embryos.
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