Effects of laser irradiation on a bloom forming cyanobacterium Microcystis aeruginosa.

Abstract

Effects of laser irradiation on photosystem II (PS II) photochemical efficiencies, growth, and other physiological responses of Microcystis aeruginosa were investigated in this study. Results indicate that laser irradiation (wavelengths 405, 450, 532, and 650nm) could effectively inhibit maximal PS II quantum yield (Fv/Fm) and maximal electron transport rates (ETRmax) of M. aeruginosa, while saturating light levels (Ek) of M. aeruginosa did not change significantly. Among the four tested wavelengths, 650nm laser (red light) showed the highest inhibitory efficiency. Following 650nm laser irradiation, the growth of M. aeruginosa was significantly suppressed, and contents of cellular photosynthetic pigments (chlorophyll a, carotenoid, phycocyanin, and allophycocyanin) decreased as irradiation dose increased. Meanwhile, laser irradiation enhanced the enzyme activities of superoxide dismutase (SOD) and peroxidase (POD) in M. aeruginosa cells. Lower irradiation doses did not change the intracellular microcystin contents, but higher dose irradiation (>1284Jcm-2) caused the release of microcystin into the culture medium. Transmission electron microscope examination showed that the ultrastructure of M. aeruginosa cells was destructed progressively following laser irradiation. Effects of laser irradiation on M. aeruginosa may be a combination of photochemical, electromagnetic, and thermal effects.