Effects of land-use intensity, grazing and fire disturbances on soil bacterial and fungal communities in subtropical wetlands

Abstract

Bacteria and fungi are primary components in wetland soil microbial communities and provide essential ecosystem functions and services. Understanding responses of bacterial and fungal communities to multiple drivers of environmental change and their interactions is crucial for wetland conservation and management, particularly for those embedded in agricultural landscapes. Yet little is known about effect of agricultural land use and wetland management on soil microbial communities in subtropical seasonal wetlands. Here, we used a long-term whole-ecosystem wetland experiment to examine individual and interactive effects of upland land-use intensification, livestock grazing, and prescribed fire on soil bacteria and fungi. We asked: (1) How do land-use intensification, grazing and fire disturbances interact to alter taxonomic composition and functional potential of wetland soil bacterial and fungal communities? (2) To what extent would these management and disturbance effects on wetland microbial communities manifest through alterations in soil properties? Our results showed that both microbial taxonomic and functional composition are responsive to agricultural land use and wetland management. Upland land-use intensification was the strongest driver (as compared to fire and grazing) in shaping bacterial and fungal community composition. Specifically, land-use intensification increased functional richness of both bacteria and fungi, whereas grazing and fire only interactively affected bacterial functional richness. In addition, responses of bacterial and fungal species diversity to wetland management varied, where grazing and fire reduced fungal species diversity in wetlands embedded in low-intensity managed pastures, but none of these management practices altered bacterial species diversity. Further, we found that pH and secondary nutrients (i.e., Ca and Mg) availability were the most important soil properties that explain how agricultural land use and wetland management drive the composition of bacterial and fungal communities. Our findings suggest that integration of lime application into intensified land uses to neutralize soil pH could facilitate maintenance of microbial diversity and associated functions. Our results highlight the need to comprehensively assess management impacts on soil microorganisms, rather than using a single or few indicators, due to inconsistent responses of bacterial and fungal communities, as well as their varied taxonomic and functional responses.