Effects of Laminaria japonica polysaccharides on exercise endurance and oxidative stress in forced swimming mouse model.

Feiwei Yan,Haitao Hao

doi:10.1186/s40709-016-0049-4

Abstract

BackgroundPolysaccharides are the major active ingredients responsible for the bioactivities of Laminaria japonica. However, the effects of L. japonica polysaccharides (LJP) on exercise endurance and oxidative stress have never been investigated. Therefore, this study was conducted to investigate the effects of LJP on exercise endurance and oxidative stress in a forced swimming mouse model. The animals were divided into four groups, namely the control (C), LJP-75, LJP-150, and LJP-300 groups, which received physiological saline and 75, 150, and 300 mg kg−1 LJP, respectively, by gavage once a day for 28 days. This was followed by a forced swimming test and measurements of various biochemical parameters.ResultsLJP increased swimming time to exhaustion, the liver and muscle glycogen content, and levels of superoxide dismutase, glutathione peroxidase, and catalase in the serum, liver, and muscle, which were accompanied by corresponding decreases in the malondialdehyde (MDA) content of the same tissues. Furthermore, decreases in blood lactic acid and serum myeloperoxidase (MPO) levels were observed.ConclusionLJP enhanced exercise endurance and protected mice against exhaustive exercise-induced oxidative stress.

Highlights

Polysaccharides are the major active ingredients responsible for the bioactivities of Laminaria japonica
Effects of L. japonica polysaccharides (LJP) on swimming time to exhaustion of mice Exercise endurance is an important parameter for evaluating anti-fatigue treatments, and the forced swimming test has been widely used for this purpose with high reproducibility [26]
Effects of LJP on glycogen contents of the liver and muscle of mice Energy for exercise is derived initially from the breakdown of glycogen in muscle which may be depleted during strenuous exercise, and at later stages, the energy is derived from the liver glycogen [10, 28]

Summary

Results

LJP increased swimming time to exhaustion, the liver and muscle glycogen content, and levels of superoxide dismutase, glutathione peroxidase, and catalase in the serum, liver, and muscle, which were accompanied by corresponding decreases in the malondialdehyde (MDA) content of the same tissues. Decreases in blood lactic acid and serum myeloperoxidase (MPO) levels were observed

Background

Results and discussion

Conclusions

Methods