Abstract
Abstract Background HAMLET (human α-lactalbumin made lethal to tumor cells) and sulindac are active ingredients which are used in the treatment of cancers. Under both in vivo and in vitro conditions, HAMLET binds to the surface of tumor cells and enters the cells. Sulindac is one of nonsteroidal anti-inflammatory drugs. It inhibits the growth of tumor cells by inducing the apoptosis. In our study, we aimed to investigate effects of α-lactalbumin and sulindac on COLO-320 primary and COLO-741 metastatic human colon carcinoma cell lines via mitochondrial apoptotic pathway by indirect immunocytochemistry and TUNEL assays. Materials and methods The effects of α-lactalbumin and sulindac were assessed by using MTT assay at 24, 48 and 72 h and apoptosis markers caspase-3, caspase-9 and cytochrome-c were detected using immunocytochemistry and TUNEL methods. Results It was appeared that α-lactalbumin and sulindac may triggger mechanisms of apoptosis in both primary and metastatic colon carcinoma cell lines and the primary colon carcinoma cell line was affected more than the metastatic cells. Conclusion It is proposed that α-lactalbumin and sulindac can be used in cancer treatments and future in vivo experiments.
Highlights
Colorectal cancer (CRC) is one of the causes of cancerrelated deaths worldwide [1, 2]
It is proposed that α-lactalbumin and sulindac can be used in cancer treatments and future in vivo experiments
Amaç: HAMLET ve sulindac, kanser tedavisinde kullanılan etken maddelerdir
Summary
Colorectal cancer (CRC) is one of the causes of cancerrelated deaths worldwide [1, 2]. Α-Lactalbumin is the most abundant protein in female human milk [8] It forms an active complex with oleic acid which is named HAMLET (human α-lactalbumin made lethal to tumor). Under both in vivo and in vitro conditions, HAMLET kills a variety of tumor cells such as human skin papilloma, colorectal and bladder cancer cells through the process of apoptosis, while healthy cells remain unaffected [5]. HAMLET (human α-lactalbumin made lethal to tumor cells) and sulindac are active ingredients which are used in the treatment of cancers. Under both in vivo and in vitro conditions, HAMLET binds to the surface of tumor cells and enters the cells. Results: It was appeared that α-lactalbumin and sulindac may triggger mechanisms of apoptosis in both primary and metastatic colon carcinoma cell lines and the primary colon carcinoma cell line was affected more than the metastatic cells
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