Abstract
The aim of this study was to evaluate the effect of L-carnitine (LC) supplemented in vitro maturation (IVM) medium on the cryotolerance and development of buffalo oocytes. Mitochondrial activity, reactive oxygen species (ROS) content, and lipid droplet distribution of vitrified-warmed buffalo oocytes were also observed. Oocytes were matured in IVM medium supplemented with 0, 0.3, 0.6, and 1.2 mg/mL LC and some of them were vitrified by the Cryotop® method. The highest survival, morula, and blastocyst rates were obtained with 0.6 mg/mL LC, irrespective of whether oocytes were fresh or vitrified-warmed. The density of active mitochondria in vitrified oocytes was significantly higher with 0.6 mg/mL LC than without LC treatment. In contrast, H2O2 levels and lipid droplet content were significantly lower following LC treatment than without it. In conclusion, supplementation with 0.6 mg/mL LC during IVM improves the buffalo oocytes’ survival rate and subsequent embryo development after in vitro fertilization. This may be linked to improved mitochondrial activity, enhanced β-oxidation, and reduced ROS levels.
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