Abstract
Problem statement: Rats fed high dietary fructose are documented to f orm an acquired model of insulin resistance; the present study aims to investigate possible changes in lens crystallin s of rats fed high fructose diet and the effects of admi nistration of each exogenous L-Carnitine (CA) and Cinnamon Extract (CE) on protein glycation, oxidati ve stress and redox homeostasis in this rat model. Approach: A total number of 60 male Wister rats of body weig ht 120-160 g were divided into 4 groups of 15 rats each. Group 1 received control di et, while groups 2, 3 and 4: rats received high fructose diet (60g/100 g diet). After 2 weeks from fructose feeding, animals of group 3 were treated with L-carnitine (300 mg g -1 body weight/day i.p.), while animals of group 4 we re treated with cinnamon extract (0.5 mL/rat/day orally). At the en d of experimental period (30 days), serum levels of glucose and insulin were determined. Lenses of each animal were dissected; molecular weights of crystalline, oxidative stress markers, early glycat ion of lens proteins and carbonyl group were assaye d. Results: A significant decline in antioxidants and increase in lipid peroxidation products, protein oxidation and protein glycation were observed in lens samples obtained from fructose-fed rats. Administration of each CA and CE to fructose-fed rats significantly attenu ated oxidative damage and protein glycation and ret urned levels of antioxidants to near those in control gro up. Chromatographic analysis of lens crystalline of rats fed high fructose diet showed diffused peaks, indicatin g crystalline aggregation. Conclusion: The results of the present study indicate that dietary fructose di sturbs lens integrity and administration of L-carni tine or cinnamon extract may safeguard the lens by minimizing the protein aggregation, preventing glycation and oxidative stress in animals fed high fructose diet. L-carnitine has more potent effects than CE in this animal model.
Highlights
(Nandhini et al, 2002). Herbert et al (1999) reported the effect of moderate and severe levels of diabetes on the optical performance of the rat lens and evaluated the effect of dietary fructose on diabetic lens damage
The fructose fed rats developed hyperinsulinemia and hyperglycemia with increased HOMA index which were prevented by carnitine and cinnamon extract
Chromatographic analysis of lens crystallins: Pools of three pairs of rat lenses were homogenized in distilled water (0.2 g lens/2.5 mL distilled water) and centrifuged in a cooling centrifuged at 8000 rpm for 20 min the supernatant was subjected to column chromatographic analysis according to the method of Testa et al (1965)
Summary
(Nandhini et al, 2002). Herbert et al (1999) reported the effect of moderate and severe levels of diabetes on the optical performance of the rat lens and evaluated the effect of dietary fructose on diabetic lens damage. Herbert et al (1999) reported the effect of moderate and severe levels of diabetes on the optical performance of the rat lens and evaluated the effect of dietary fructose on diabetic lens damage. Animals were divided into the biochemical function of CA is the transport of long- following groups consisting of 15 rats for each: chain fatty acids across the inner mitochondrial membrane into the matrix for β-oxidation and has effects on oxidative metabolism of glucose in tissues (Broderick et al, 1992; Rajasckar et al, 2006). Group 1 (CON): animals received the control diet and tap water ad libitum. The control diet contained corn starch (60%) as the sole source of carbohydrate, 20% casein, 0.7% methionine and 5% ground nut oil, 10.5% wheat bran and 3.5% salt mixture. Vitamin mixture (0.2 mL) was added per one kilogram feed
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Biochemistry and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
