Effects of L-carnitine against H2O2-induced oxidative stress in grass carp ovary cells (Ctenopharyngodon idellus).

Abstract

This study was designed in vitro to investigate the effects of L-carnitine against H2O2-induced oxidative stress in a grass carp (Ctenopharyngodon idellus) ovary cell line (GCO). GCO cells were pre-treated with different concentrations of L-carnitine, followed by incubation with 2.5mM H2O2 for 1h to induce oxidative damage. The results indicated that adding L-carnitine at concentrations of 0.01-1mM into the medium for 12h significantly increased cell viability. Pre-treatment with L-carnitine at concentrations of 0.1-5mM for 12h significantly inhibited 2.5mM H2O2-induced cell viability loss. The significant decreases in the level of reactive oxygen species and cell apoptosis were observed in 0.5mM L-carnitine group compared to the H2O2 group. Malondialdehyde values of all of the L-carnitine groups were significantly lower than those of the H2O2 group, while total glutathione levels of all of the L-carnitine groups were significantly higher than of the H2O2 group. The activity of antioxidant enzymes, such as total superoxide dismutase (0.1 and 0.5mM L-carnitine), catalase (0.5mM L-carnitine) and γ-glutamyl cysteine synthetase (0.5 and 1mM L-carnitine), was significantly increased. In addition, pre-treatment of L-carnitine in GCO cells exposed to 2.5mM H2O2 significantly increased the mRNA expression of copper, zinc superoxide dismutase, catalase (0.5mM L-carnitine), glutamate cysteine ligase catalytic subunit (0.1-1mM) and glutathione peroxidase (0.1mM L-carnitine). In conclusion, L-carnitine promotes GCO cell growth and improves antioxidant function, it plays a protective role against oxidative stress induced by H2O2 in GCO cells, and the appropriate supplemental amount of L-carnitine is 0.1-1mM.