Effects of Kuntai Capsules on endometrial thickness and expressions of leukemia inhibitory factor and epidermal growth factor in mouse after controlled ovarian hyperstimulation

Abstract

To explore the effects of Kuntai Capsules on endometrial thickness and the expressions of leukemia inhibitory factor (LIF) and epidermal growth factor (EGF) in mouse after controlled ovarian hyperstimulation. Healthy Sprague-Dawley mice were randomly allocated into 4 groups of control (group A), controlled ovarian hyperstimulation [COH (group B)], COH plus low-dose Kuntai Capsules (group C) and COH plus large-dose Kuntai Capsules [2x low-dose (group D)]. The controlled ovarian hyperstimulation model was established. The endometrial thickness was measured by computerized multi-functional image analyzer. And the expressions of LIF and EGF in proliferating endometrium were examined by immunohistochemistry. The endometrial thickness of groups C and D were higher than that of groups A and B. And there were significant differences (P < 0.05). The expression levels of LIF protein in proliferating endometrium was weaker than implantation window phase, the expression levels of EGF and LIF in group B was weaker than group A, groups C and D stronger than groups B and D was stronger than group C. And there were significant differences (P < 0.05). Kuntai Capsules can promote the growth of endometrium and enhance the expression levels of EGF and LIF in mice. And it may improve the ability of endometrial receptivity through optimized microenvironment. And a larger dose of Kuntai Capsules yields better outcomes.