Effects of Kudiezi injection on proliferation and apoptosis of human lung cancer cell line A549

Abstract

Objective To observe the effect of Kudiezi injection (KDI)on proliferation and apoptosis of human lung cancer cell line A549.Methods After administration of 12.5-400.0 g/L KDI for 24-72 h,the methyl thiazol tetrazolium (MTT) method was used to investigate the inhibitory effect of KDI on A549 cells.Cell apoptosis and cell cycle arrest were investigated by flow cytometry (FCM).Western blot was used to detect the expression of p53 protein.Results KDI significantly inhibit the growth of A549 cancer cells in dose-dependent manners.The number of G0/G1 stage cell was (75.15 ± 1.00)％,(77.52± 1.09) ％,( 79.70 t 1.48 ) ％ and ( 74.55 ± 0.91 ) ％,significant higher than constrol group ( 66.28 ±1.91 )％ (P ＜0.01 ),and that of p53 expression of A549 cancer cells was higher than it in constrol group (P ＜0.05).Conclusion These findings suggest that KDI can inhibit the proliferation of human lung cancer cell line A549 and have obvious dose-effect relationship.The drug inducing people A549 lung cancer cell apoptosis may be relevant to promoting p53 protein expression and its block cells from G1 to S period. Key words: Lung cancer; Kudiezi injection; Proliferation; Apoptosis; Cell cycle; p53