Abstract

Using two cDNA clones which represent the 82 kDa and 76 kDa larval hemolymph protein (LHP) genes of Galleria mellonella, the effects of juvenile hormone (JH), 20-hydroxyecdysone (20-HE) and nutrition on the levels of the correspoding transcripts in last instar larvae were determined. The transcripts coding for LHP82 and LHP76 declined in intact day-2 or day-3 last instar larvae 72–96 hr after application of 20 μg of JH, but in prothorax-ligated day-3 larvae JH had no effect on the levels of these transcripts. Application of 5 μg of 20-HE either to intact or to prothorax-ligated day-3 last instar larvae caused a decline in the levels of both the transcripts in 30–60 hr. Starvation of larvae beginning 24 or 48 hr after the last larval ecdysis did not inhibit production of either of the LHP transcripts, but starvation during the first 24 or 48 hrs of the last larval stadium, followed by normal feeding, delayed production of the LHP transcripts for up to 6 days. Ligation of the starved larvae 2 or 3 days after resumption of feeding accelerated production of the LHP76, but not LHP82, complementary transcripts. These data suggest that the two LHP genes are independently regulated, and that both are repressed by ecdysteroids.

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