Effects of isolated nonspecific binders upon the search for specific targets: Absolute rates versus competition between the targets.

Abstract

Many biological processes involve macromolecules searching for their specific targets that are surrounded by other objects, and binding to these objects affects the target search. Acceleration of the target search by nonspecific binders was observed experimentally and analyzed theoretically, for example, for DNA-binding proteins. According to existing theories this acceleration requires continuous transfer between the nonspecific binders and the specific target. In contrast, our analysis predicts that (i) nonspecific binders could accelerate the search without continuous transfer to the specific target provided that the searching particle is capable of sliding along the binder; (ii) in some cases such binders could decelerate the target search, but provide an advantage in competition with the "binder-free" target; (iii) nonbinding objects decelerate the target search. We also show that although the target search in the presence of binders could be considered as diffusion in inhomogeneous media, in the general case it cannot be described by the effective diffusion coefficient.