Abstract
This study aims to investigate the effects of gamma irradiation on antimicrobial activities (against Staphylococcus aureus and Escherichia coli) and antioxidant activities of Coscinium fenestratum (Goetgh.) Colebr., known in Thai as Haem. Gamma irradiation was applied to powder samples of Haem at 5, 10, 15 and 20 kGy. The 60% ethanolic extracts of irradiated and non-irradiated samples were prepared to evaluate total phenolic contents (TPC), antioxidant and antimicrobial activities. Results indicated that TPC of irradiated and non-irradiated samples was not significantly different (p<0.05), as the TPC values of all samples ranged from 11.39± 1.17 to 11.99±1.10 mgGAE/g. Ferric reducing antioxidant potential and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity significantly increased (p<0.05) after gamma irradiation at 20 kGy and 5 kGy, respectively. The antimicrobial activity was done using agar disc diffusion method. Results showed that ethanolic extracts of various samples exhibited antibacterial activities against S. aureus as evidence by the clear zone, 16.3 – 17.5 mm, that formed around the disc samples.
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