Effects of Irisin on proliferation, apoptosis, migration and invasion of human cholangiocarcinoma cell line Hucct-1

Abstract

Objective To investigate the effect of Irisin on proliferation, apoptosis, migration and invasion of human cholangiocarcinoma cell line Hucct-1. Methods After treatment with Irisin, CCK-8 assay and flow cytometry assay were conducted to investigate the effect of Irisin on proliferation and apoptosis of cholangiocarcinoma cells. Scratch test and transwell invasion assay were used to studythe effect of Irisin on the migration and invasion ability of cholangiocarcinoma cells. Western blot was utilized to detect the expression of E-cadherin, N-cadherin and Vimentin in cholangiocarcinoma cells. Results CCK-8 assay showed that Irisin inhibited cholangiocarcinoma cell proliferationin a dose-dependent manner. Flow cytometry assay showed that the apoptosis rate of Irisin group [(14.8±0.9)%] was higher than that in the control group [(5.4±0.6)%], (P<0.05). The scratch test showed that the rate of cell scratch healing in Irisin group [(15.0±1.0)%] was significantly lower than that in the control group [(28.0±2.0)%] (P<0.05). Transwell invasion test showed that the number of cells in Irisin group was (96.0±7.0), which was significantly lower than that in control group (155.0±9.0) (P<0.05). Western blot showed that the expression of E-cadherin increased and N-cadherin and Vimentin decreased after Irisin treatment. Conclusion Irisin inhibits proliferation, migration and invasion and promote apoptosis of cholangiocarcinoma cells. Key words: Intrahepatic cholangiocarcinoma; Irisin; Proliferation; Apoptosis; Migration; Invasion