Abstract
Objective To explore the effects of Iptakalim on intracellular free calcium concentration and on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1(ET-1) in vitro. Methods A cell culture model, [ 3H]-thymidine([ 3H]-TdR) incorporation test and confocal microscope were used to observe proliferation and intracellular free calcium concentration([Ca 2+] i) of rabbit PASMC induced by ET-1 in vitro. Results The value of [ 3H]-TdR incorporation in ET-1 group was increased 1.468 times higher than that in control group. Iptakalim at the concentration of 10 −7mol/L, 10 −6 mol/L, 10 −5 mol/L lowered [ 3H]-TdR incorporation by (19.8 ± 4.6)%, (41.2 ± 9.5)%, (54.7 ± 10.1)%, respectively, compared with the value of the cells treated with ET-1( P < 0.01); The intracellular fluorescence intensity of PASMC in ET-1 group was increased from 73.70 ± 10.12 to 143.84 ± 28.23, significantly higher than that in control group( P < 0.01); whereas with Iptakalim, the fluorescence intensity(FI) was only increased from 74.30 ± 10.20 to 86.03 ± 9.82, significantly lower than that in ET-1 group( P < 0.01). Conclusion Iptakalim inhibited proliferation of PASMC and decreased intracellular free calcium concentration of cultured rabbit PASMC induced by ET-1.
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