Effects of iontophoretically applied acetylcholine on membrane potential and synaptic activity of bulbar respiratory neurones in decerebrate cats

Abstract

Intracellular recordings were made from bulbar respiratory neurones of decerebrate cats, together with iontophoretic application of acetylcholine and its specific antagonists to assess cholinergic mechanisms involved in the central respiratory network in vivo. Of 126 respiratory-related neurones impaled in the ventral respiratory group, iontophoresis of acetylcholine produced depolarization in 67 cells (53%), hyperpolarization in 36 cells (29%), and no effect in the remaining 23 cells (18%). Depolarization occurred predominantly in laryngeal motoneurones ( 31 40 ) and bulbo-spinal neurones ( 4 5 ), while a comparable number of non-antidromically-activated respiratory neurones displayed either depolarization ( 33 81 ) or hyperpolarization ( 31 81 ). Acetylcholine had no significant effect on excitatory and inhibitory postsynaptic potentials in all types of neurones tested. Both depolarizing and hyperpolarizing effects of acetylcholine were antagonized by coiontophoresis of atropine, but not by hexamethonium. Input resistance was increased ( 7 9 ) or unchanged ( 2 9 ) in depolarized cells, whereas it was unaltered in all hyperpolarized cells tested ( n = 6). The present results suggest that the distribution and functions of cholinergic muscarinic receptors are different for the laryngeal and bulbo-spinal types of respiratory neurones and the non-antidromically-activated respiratory neurones in the cat.