Abstract
Ionic liquid (IL) has attracted wide attention as a pretreating solvent for efficient utilization of lignocellulosic biomass. However, IL is known to severely inactivate the cellulases. In this study, the relative activity of Paenibacillus sp. LLZ1 cellulases (PCs) was analyzed in the presence of 1-ethyl-3-methylimidazolium diethylphosphate ([Emim]DEP) with respect to carboxymethylcellulose (CMC), filter paper (FP), and microcrystalline cellulose (MCC). With increasing [Emim]DEP concentration, PCs were differentially inhibited depending on the substrate. Subsequently, the kinetic parameters Km and Vmax were analyzed. A significant increase in the Km indicated that [Emim]DEP exerted a negative impact on the affinity of the enzyme on the substrate MCC. Comparatively, the Vmax was influenced only slightly. Further study on the relative MCCase activity with different substrate content showed that PCs were less inhibited at higher substrate concentration. Finally, sodium dodecyl sulfate polyacrylamide gel elec...
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