Abstract
The hydrodynamic shape, conformation, and thermal stability of ribosomal 23S RNA have been studied by sedimentation velocity analysis, circular dichroism, ultraviolet absorption, and thermal denaturation. The results show that magnesium ion is critically required for maintaining the structural integrity of ribosomal 23S RNA. Upon removal of magnesium ions, the sedimentation coefficient of 21.1 S for native 23 S RNA is reduced to approximately 8.5 S, indicative of a large unfolding of the RNA. A large increase in absorbance at 260 nm in UV spectra of 23S RNA and a decrease in ellipticity near the 265 nm peak which was followed by parallel changes in the 237 nm trough and the 208 nm trough. Furthermore, the melting temperature (Tm) of the 23S RNA is lowered to 50°C from 65°C with a concomitant decrease in the cooperativity of the melting curve of 23S RNA.
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