Effects of intrauterine exposure to parathion on the activity of renal ATPases in offspring

Abstract

The effects of ethyl parathion on the activities of various renal enzymes were studied in the offspring from dams treated with this insecticide during pregnancy. The enzymes tested were the (Na+-K+)- and the Mg2+-dependent ATPases, the glutathione S-transferases and carboxylesterases. The postnatal effects of parathion on kidney ATPases from undernourished rats were also assessed. The organophosphate was administered per os to pregnant rats at a dose of 1 mg kg-1 body weight per day throughout gestation, and suspended after delivery. The offspring were divided in groups of normally-fed and undernourished rats. In the undernourished group, food restriction produced a decrease of 43% in body weight as compared to the normally-fed group. Offspring were sacrificed 6 weeks after birth and the enzymatic activities were determined in kidney homogenates. We found a decrease in the enzymatic activity of total ATPases, at the expense of the Mg2+-dependent ATPase. However, the activities of the (Na+-K+)-dependent ATPase, the glutathione S-transferases and the carboxylesterases did not show significant changes. On the other hand, undernutrition did not potentiate the effects of parathion on the ATPases. Thus, this organophosphate administered during pregnancy produced a selective inhibition on the renal Mg2+-dependent ATPase from offspring, which was not potentiated by our undernutritional model.