Abstract
The effect of gold nanoparticles on lung cancer cells is not yet clear. In this study, we investigated the cytotoxicity and cell invasion activity of lung cancer cells after treatment with gold nanoparticles and showed that small gold nanoparticles can be endocytosed by lung cancer cells and that they facilitate cell invasion. The growth of A549 cells was inhibited after treatment with 5-nm gold nanoparticles, but cell invasion increased. Endocytosed gold nanoparticles (size, 10 nm) notably promoted the invasion activity of 95D cells. All these effects of gold nanoparticles were not seen after treatment with larger particles (20 and 40 nm). The enhanced invasion activity may be associated with the increased expression of matrix metalloproteinase 9 and intercellular adhesion molecule-1. In this study, we obtained evidence for the effect of gold nanoparticles on lung cancer cell invasion activity in vitro. Moreover, matrix metalloproteinase 9 and intercellular adhesion molecule-1, key modulators of cell invasion, were found to be regulated by gold nanoparticles. These data also demonstrate that the responses of the A549 and 95D cells to gold nanoparticles have a remarkable relationship with their unique size-dependent physiochemical properties. Therefore, this study provides a new perspective for cell biology research in nanomedicine.
Highlights
Previous studies identified that gold nanoparticles (Au-NPs) show little cytotoxicity despite their efficient uptake into human cells by endocytosis [1,2], making them suitable candidates for nanomedicine
Au-NPs are used in different biomedical applications: can they be used as scaffolds for increasingly potent cancer drug delivery but they can serve as transfection agents for selective gene therapy and as intrinsic antineoplastic agents[7,8,9]
Internalization of Au-NPs To investigate whether Au-NPs with various sizes crossed the cell membrane and where they located, A549 and 95D cells were incubated for 48 h in the presence of Au-NPs (5, 10, 20, and 40 nm) in complete cell culture medium
Summary
Previous studies identified that gold nanoparticles (Au-NPs) show little cytotoxicity despite their efficient uptake into human cells by endocytosis [1,2], making them suitable candidates for nanomedicine. Besides their biocompatibility, the fact that they are easy to synthesize, characterize, and surface modify contributed to attract much attention in various biomedical applications. Au-NPs are used in different biomedical applications: can they be used as scaffolds for increasingly potent cancer drug delivery but they can serve as transfection agents for selective gene therapy and as intrinsic antineoplastic agents[7,8,9]. Dreaden et al [8] have shown that targeted Au-NPs are capable of altering the cell cycle, including cell division, signaling, and proliferation
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