Abstract
Aim: To investigate the changes of A549 protein 6PGD expression, tumor growth and migration, NADPH and ROS levels in lung cancer cells after intermittent intervention with DDP. Methods: The sensitivity of A549 cells to DDP was detected by cck-8 method, A549 cells were treated by DDP intermitten, 6PGD protein expression was detected by Western blot method, and the ROS level of A549 cells was determined by ROS kit. NADPH levels of A549 cells were determined by NADPH kit. Result: After DDP intermittent intervention, the expression of 6PGD protein in lung cancer cells A549 was increased, the growth and migration of A549 cells were significantly inhibited, and ROS and NADPH levels were significantly increased. Conclusion: 6PGD were upregulated in after DDP intermittent intervention and affected the migration ability in lung cancer cells.
Highlights
Lung cancer is one of the most common malignant tumors with the highest morbidity and mortality
A549 cells were treated with DDP to produce drug resistance, and the 6PGD protein level, reactive oxygen species (ROS) and NADPH changes of A549 cells were observed, and their effects on cell growth and migration were observed
Western blot to detect the expression of 6PGD protein level: A549 cells treated with intermittent intervention with DDP concentration of 0 mg/L, 0.4 mg/L, 0.8 mg/L, and 1.2 mg/L were collected, and protein content was determined by BCA method
Summary
Lung cancer is one of the most common malignant tumors with the highest morbidity and mortality. The occurrence of cell migration in lung cancer tissue is one of the main reasons why it is difficult to cure and increase mortality[25]. Glucose 6phosphate dehydrogenase (6PGD) is one of the main enzymes in the pentose phosphate pathway (PPP). One of the important sources of products of the pathway, provides reducing energy for biosynthesis, and can offset the toxicity of reactive oxygen species (ROS) to cells, so it is a very promising anti-cancer target[8]. A549 cells were treated with DDP to produce drug resistance, and the 6PGD protein level, ROS and NADPH changes of A549 cells were observed, and their effects on cell growth and migration were observed
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