Abstract
Objective To analyze the effect of interleukin (IL)-21 on induction of cytokine-induced killer cells (CIK) from human peripheral blood mononuclear cells (PBMCs) in vitro.Methods CIKs were induced from PBMCs of healthy volunteers.The control group was only given conventional medium,and IL-21 was given in IL-21 experimental group.The cells were cultured in an incubator with 5％CO2 at 37 ℃ for 10 days.The proliferation of CIK was analyzed by cell counting trypan blue exclusion test.Fluorescence labeled antihuman CD3,CD4,CD8 and CD56 + antibodies were used for FCM analysis.The cytokines in the culture medium produced by CIK,including interferon (IFN)-γ,tumor necrosis factor (TNF)-α and IL-12,were analyzed by enzyme-linked immunosorbent assay (ELISA).Cytotoxcity on esophageal cancer cells (EC9706) was analyzed by cell counting kit-8 (CCK-8) and apoptosis assays.Results Cell density in IL-21 experimental group was (14.58 ± 1.81) × 108/L,greater than that in control group (7.83 ± 1.18) × 105/L (P ＜ 0.05).In IL-21 experimental group,proportion of CD3 + cells was (98.48 ± 0.28) ％,higher than that in control group [(95.02 ± 1.18) ％] (P ＜ 0.05),and that of CD3+CD4+ was (51.53±1.21)％,higher than in control group [(33.36±1.91)％] (P＜ 0.05).No obvious difference in the proportion of CD3 + CD8 + or CD3 + CD56 + was found between two groups.The concentration of TNF-α in CIK culture medium in IL-21 experimental group was 25.0 μg/L,higher than 12.5 μg/L in control group (P ＜0.05),but there was no significnat difference in IFN-γ concentration.Apoptosis rate was (15.33 ± 0.91) ％ in IL-21 experimental group,higher than (13.25 ± 1.17) ％ in control group (P ＜ 0.05).Conclusion IL-21 can promote the proliferation of CIK.It can increase the proportion of CD3 +,and CD3 + CD4 + in CIK.IL-21 can increase the TNF-o production in CIK,promoting the apoptosis of tumor cells. Key words: Interleukin-21 ; Cytokine-induced killer cells; Apoptosis
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