Abstract
Bacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco. Integrated biocontrol method was explored to control bacterial wilt. Nevertheless, the long-term effects of the integrated biocontrol method on soil bacterial community, soil physicochemical properties and the incidence of bacterial wilt are not well understood. In this study, B. amyoliquefaciens ZM9, calcium cyanamide and rice bran were applied to tobacco fields in different ways. The disease index and incidence of tobacco bacterial wilt (TBW), soil physicochemical properties, colonization ability of B. amyoliquefaciens ZM9, and rhizopshere bacterial community were investigated. The results showed that the integrated application of B. amyoliquefaciens ZM9, rice bran and calcium cyanamide had the highest control efficiency of TBW and bacteria community diversity. Additionally, the integrated biocontrol method could improve the colonization ability of B. amyoliquefaciens ZM9. Furthermore, the integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil. This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW.
Highlights
Bacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco
Our previous study showed that B. amyloliquefaciens ZM9 as an antagonistic bacterium of bacterial wilt was isolated through its ability to colonize tobacco and rhizosphere soil, which can improve the microbial abundance of rhizosphere s oil[5]
The control efficacy was significantly higher in the RCFC group than in the LSFC, RBFC and CSFC groups at vigorous, budding, maturity and harvesting stages (Fig. 1)
Summary
Bacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco. The integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW. Organic fertilizers or soil amendments assistance of antagonistic bacteria should be considered as an effective integrated biocontrol method to improve the colonization ability of antagonistic bacteria and control bacterial wilt. The main aims of this study were to (1) compare the control efficacy of different treatment groups for TBW, (2) assess the colonization ability of B. amyloliquefaciens ZM9, and (3) explore the dynamic changes of physicochemical properties and rhizosphere bacterial community.
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