Abstract
Objective To investigate the effect of exogenous insulin-like growth factor-1(IGF-1)on growth of gastric smooth muscle cells(SMCs)and synthesis of stem cell factor(SCF).Methods Gastric SMCs were cultured by enzymolysis and were identified by α-actin immunocytochemical method.After that,SMCs were incubated with different concentrations of IGF-1(0,50,100,150μg/L)or IGF-1 receptor monoclonal antibody(0.50,100,150μg/L)for 24 hours.Proliferation of SMCs was measured by MTT.The expressions of SCF protein and RNA were analyzed by Western blotting and quantitative reverse transcription-polymerase chain reaction,respectively.The SCF content in the culture fluid was detected by enzyme-linked immunosorbent assay(ELISA).Results The proliferation of gastric SMCs and the expression of SCF can be enhanced by IGF-1,and 100μg/L of IGF-1 may be the final effective concentration.Nevertheless,these could be inhibited by IGF-1 receptor monoclonal antibody in a dose-dependent manner.ConclusionElevated expression of SCF may be induced by IGF-1 through proliferation of SMC. Key words: Insulin-like growth factor-1; Myocytes,smooth muscle; Stem cell factor Antibodies
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