Abstract

This study was conducted at the College of Agriculture – University of Anbar, Iraq. From 16 January to 5 February 2022, this study aimed to investigate the effect of injected egg hatching at different sialic acid times in growth and embryonic development. Four hundred eggs of hatching types (Ross 308) were injected with different sialic acid concentrations at 0 days (before placing in the incubator), 7 and 14 days of incubation. Eggs were divided into four groups (100 eggs each) as follows: T1: The control group was placed in the incubator without injection. T2: Injected with a dose of 100 μg sialic acid at the age of zero. T3: Injected with 100 μg sialic acid dose at 7 days. T4: Injected with 100 μg sialic acid dose at 14 days of incubation. Statistical analysis was performed (CRD) (P=0.05); results show: Increase in the e length of the embryo, the diameter of the vascular region and the number of pairs of somites at 3 days of incubation for T1. Increase in the percentage of embryonic weight, decrease in the percentage of Albumin and the percentage of shell at 7 days of incubation for T2 and T3. Increase in percentage of embryonic weight and amniotic sac and liquid, decrease in the percentage of Albumin and yolk, at 14 days of include sialic acid for T2. Increase the percentage of embryonic weight, and decrease the percentage of yolk at 14 days incubation for T2. They have concluded that In-Ovo injection of the hatching eggs with sialic acid contributed to increased physiological traits and embryonic development. Keywords: In-Ovo, Sialic Acid, Erythropoietin, Oxygen, Anemia, and Anoxia.

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