Effects of inorganic iodide on thyroid stimulation in vitro.

Abstract

Both pituitary thyrotropin (TSH) and the long acting thyroid stimulator (LATS) of Graves’ disease stimulate glucose oxidation and 32P incorporation into phospholipids in thyroid slices. Since inorganic iodide has been shown to stimulate intermediary carbohydrate metabolism in thyroid slices, studies were done to determine whether the effects of TSH and/or LATS on thyroidal glucose oxidation and phospholipogenesis were modified by the presence of iodide in the medium. Nal in concentrations (1.7 × 10−3m, 3.3 × 10−4m) which either stimulated or were without effect on oxidation of glucose-l-14C or 32P incorporation into phospholipids by sheep thyroid slices consistently inhibited TSH-induced stimulation of thyroidal glucose oxidation and phospholipogenesis. The same concentrations of Nal did not affect, and in a few instances augmented, LATS-induced stimulation of thyroidal glucose oxidation and 32P incorporation. Increased glucose oxidation in dog thyroid slices was observed when the gland was removed after ...