Effects of inoculation with lignocellulose-degrading microorganisms on nitrogen conversion and denitrifying bacterial community during aerobic composting

Abstract

The present study compared the effects of inoculation (WSD treatment) and non-inoculation (CK treatment) with lignocellulose-degrading microorganisms on nitrogen conversion, nitrogen functional genes, and the denitrifying bacterial community during aerobic composting, and their potential relations to NH3 and N2O emissions were also explored. Results showed that, WSD reduced the NH3 and N2O emissions by 25.9% and 34.98%, respectively, compared with CK. WSD also reduced the abundances of nitrifying (bacteria amoA) and denitrifying (nirS, nirK, and nosZ) genes during composting, which were significantly positively correlated with N2O emissions (P < 0.01). The most important nosZ denitrifying microorganisms belonged to Proteobacteria. Redundancy analysis showed that environmental factors could affect the succession of the denitrifying bacterial community during composting. Based on these results, structural equation modeling demonstrated that the reduction in N2O emissions under WSD was related to the lower accumulation of NO3–-N utilized by denitrifying microorganisms during the compost maturation period.