Effects of inhibitory substance P, capsaicin and anti-basic fibroblast growth factor antibody on collagen deposition of excision scar formation in mice

Abstract

ObJective To observe the effects of inhibitory substance P (capsaicin) and anti-bas-ic fibroblast growth factor antibody on collagen deposition during excision scar formation and explore their effects on scar formation.Methods The excision injury model on the back of mice skin was developed,and the different final concentrations of capsaicin (20 and 80 μg/0.1 ml), anti-basic fibroblast growth fac-tor antibody (20 and 80 μg/0.1 ml),or capsaicin (20 μg/0.1 ml) in combination with anti-basic fibro-blast growth factor antibody (20 μg/0.1) were used in the excision injury.Results The high dose of capsaicin (80 μg/0.1 ml) or high anti-basic fibroblast growth factor antibody (80 μg/0.1 ml) markedly inhibited collagen deposition in the excision site, but low dose of capsaicin (20 μg/0.1) or anti-basic fi-broblast growth factor antibody (20 μg/0.1) alone did not influence collagen deposition significantly.However,the combined use of low dose of capsaicin (20 μg/0.1 ml) and low dose of anti-basic fibroblast growth factor antibody (20 μg/0.1) decreased collagen deposition significantly.Conclusion The colla-gen deposition of the excision site in mice could be inhibited by capsaicin or bFGF antibodies in a dose-de-pendent manner.Combined use of capsaicin and anti-bFGF antibody could jointly inhibit scars formation during wound healing. Key words: Hypertrophic scar; Capsaicin; bFGF; Antibody; Collagen