Effects of inhibition of the GABAergic systems by picrotoxin on retention of a nociceptive experience in the rat, with special reference to the influence of cerebellar cortex output

Abstract

Adult cerebellectomized and noncerebellectomized DA/HAN strain (pigmented) female rats were submitted to a one-trial passive avoidance conditioning procedure consisting in associating darkness with a nociceptive stimulus. Seven days later, they were tested again to assess the retention stage. The results demonstrate that in noncerebellectomized rats, picrotoxin, whatever the dose, administered prior to the retention test, does not significantly impair retrieval. On the contrary, when administered just prior to the initial conditioning, impairments of the initial single nociceptive experience were evident (the greater the picrotoxin dose, the greater the impairments). In animals that were cerebellectomized 1 week before the experiment, picrotoxin administered at a low dose before the initial experience elicited memory impairments that were similar to those induced in noncerebellectomized rats but that were greater than those elicited in cerebellectomized, nontreated animals. However, in cerebellectomized rats, picrotoxin administered at a low dose elicited memory impairments that were weaker than in noncerebellectomized animals injected with a high dose of the drug. Considering that a low dose of picrotoxin administered to cerebellectomized animals had effects that were similar to those of a high dose injected to noncerebellectomized rats, and given that it has previously been demonstrated that a cerebellectomy performed after a single nociceptive experience impairs its memory, it is tempting to suggest that the two different doses of the drug administered to cerebellectomized and noncerebellectomized rats have similar effects on memory. If such an interpretation is valid, the information would have to leave the cerebellar cortex to be stored for long.