Effects of inhibition of glutamate uptake on the electrophysiological, optical and neurochemical characteristics of low-[Mg2+]-induced epileptiform activity in juvenile rat hippocampal slices

Abstract

Event Abstract Back to Event Effects of inhibition of glutamate uptake on the electrophysiological, optical and neurochemical characteristics of low-[Mg2+]-induced epileptiform activity in juvenile rat hippocampal slices Gabriella Nyitrai1*, Bálint Lastóczi1, Pál Szabó1 and Julianna Kardos1 1 Chemical Research Center, Hungarian Academy of Sciences, Department of Neurochemistry, Institute of Biomolecular Chemistry, Hungary Although various data support a role for the ambient Glu played in epilepsy, patterns associated with the cellular uptake of Glu have not been addressed in detail. We compared the effects of L-trans-pyrrolidine-2,4-dicarboxylate (tPDC), DL-threo-b-benzyloxyaspartate (DL-TBOA) and dihydrokainate (DHK) representing different transporter specificity and membrane-permeability profiles on recurrent seizure-like events (SLEs) evoked in hippocampal slices by low-[Mg2+] condition. In the presence of these inhibitors, the onset time and the amplitude of the first occurring SLE decreased. Uptake inhibitors frequently aborted SLEs sequential to the first occurring one. SLE duration either increased or decreased depending on the type and the concentration of the particular inhibitor applied. Simultaneous field potential and whole-cell current measurements indicated depolarization-induced inactivation of CA3 pyramidal neurons recorded after the development of the first SLE in the presence of the higher concentrations of the inhibitors (DHK 300 µM; DL-TBOA 50 µM; tPDC 500 µM). Simultaneous measurements of intrinsic optical signal (IOS) also supported prolonged neuronal activation during SLEs. Neurochemical correlates were monitored during Glu uptake inhibition or SLEs through the analysis of perfused ACSF samples in combination with the use of HPLC-coupled tandem mass spectrometry (MS/MS) detection. In conclusion, our findings suggest a role for Glu transport in the genesis and maintenance of recurrent epileptiform discharges. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Disorders of the nervous system Citation: Nyitrai G, Lastóczi B, Szabó P and Kardos J (2010). Effects of inhibition of glutamate uptake on the electrophysiological, optical and neurochemical characteristics of low-[Mg2+]-induced epileptiform activity in juvenile rat hippocampal slices. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00053 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Apr 2010; Published Online: 20 Apr 2010. * Correspondence: Gabriella Nyitrai, Chemical Research Center, Hungarian Academy of Sciences, Department of Neurochemistry, Institute of Biomolecular Chemistry, Budapest, Hungary, nyitraig@chemres.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gabriella Nyitrai Bálint Lastóczi Pál Szabó Julianna Kardos Google Gabriella Nyitrai Bálint Lastóczi Pál Szabó Julianna Kardos Google Scholar Gabriella Nyitrai Bálint Lastóczi Pál Szabó Julianna Kardos PubMed Gabriella Nyitrai Bálint Lastóczi Pál Szabó Julianna Kardos Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.