Abstract
The present study has been performed to investigate how PGs would participate the hatching process. Effects of indomethacin, an antagonist to PGs biosynthesis, on the hatching of mouse blastocysts were examined in vitro. Furthermore, it was studied that prostaglandin E 2 (PGE 2), prostaglandin F 2α (PGF 2α) or 6-keto-prostaglandin F 1α (6-keto-PGF 1α) were added to the culture media with indomethacin. (1) The hatching was inhibited by indomethacin yet the inhibition was reversible. (2) In the groups with indomethacin and PGE 2, no improvement was seen in the inhibition of hatching and the inhibition was irreversible. (3) In the groups with indomethacin and PGF 2α, inhibition of hatching was improved in comparison with the group with indomethacin. (4) In the groups with indomethacin and 6-keto-PGF 1α, no improvement was seen. The above results indicated that PGF 2α possibly had an accelerating effect on hatching and a high concentration of PGE 2 would exert cytotoxic effect on blastocysts.
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