Effects of Indole-3 acetic acid and turnip peroxidase and Indole-3 acetic acid and horseradish peroxidase systems on human colon cancer HT2G cells

Abstract

The addition of cholesterol into the AOT/isooctane/H2O system makes the surfactant monolayer of the reverse micellar droplets rigid with concomitant decrease of interdroplet interactions. So a peroxidase molecule hosted by some of these droplets experiences a completely different atmosphere than that without the additive. The change of composition of the reverse micelles results in enhancing the catalytic activity of the enzyme at various temperatures and pH values. A different trend of variation of molar absorption coefficient (ϵ) is also found Wo ≅ 10, where ϵ gradually decreases with a rise in Wo value till Wo ≅ 10 and above which it remains more or less constant. There is no shift of pH profile in the micellar system by the introduction of the cholesterol into it. By measuring the catalytic constant (kcat) it is found that the critical Wo shifts from 12 to a value 10.